Protein Targeting Protocols

Name: Protein Targeting Protocols
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Bol Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins. In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it. Expert researchers describe the key methods for detecting and analyzing proteins and protein motifs involved in targeting interactions, mostly from the perspective of intracellular signaling. The techniques include those designed to reveal the existence of targeting interactions and characterize their physico-chemical properties, to identify and isolate interacting proteins on the basis of the bait-prey' principle, to target domains, and to modify lipids. The methods are richly detailed for use by those researchers interested in defining and characterizing the protein protein interactions that underlie targeting mechanisms in all areas of molecular cell biology.

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Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins. In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it. Expert researchers describe the key methods for detecting and analyzing proteins and protein motifs involved in targeting interactions, mostly from the perspective of intracellular signaling. The techniques include those designed to reveal the existence of targeting interactions and characterize their physico-chemical properties, to identify and isolate interacting proteins on the basis of the bait-prey' principle, to target domains, and to modify lipids. The methods are richly detailed for use by those researchers interested in defining and characterizing the protein protein interactions that underlie targeting mechanisms in all areas of molecular cell biology.

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