Preparative Chromatography Techniques

Purified natural products are required for complete spectro­ scopic identification and full characterization of new compo­ unds, for biological testing and for the supply of pharmaceuti­ cals, standards, and starting materials for synthetic work. This book describes modern preparative chromatography techniques and their application in the separation of natural products, synthetic intermediates, metabolites, macromolecules, and biologically active substances, e.g. new lead compounds from microorganisms and both marine and terrestrial higher organisms. The authors focus their book on the applications and aim to provide the reader with a rapid idea on how to obtain milligram or gram quantities fo pure constituents from complex mixtures with minimum effort. Among others different pressure LC mehtods, including preparative HPLC and medium-pressure LC are treated and new all-liquid techniques, e.g. centrifugal partition chromatography, are discussed in detail. Descriptions of apparatus and operation procedures together with numerous examples of actual separations - often widely scattered in literature - are provided. This completely revised second edition has been enlarged by chapters on macromolecule and enantiomer separation and on preparative GC. This book describes modern preparative chromatography techniques and their application in the separation of natural products, synthetic intermediates, metabolites, macromolecules, and biologically active substances, e.g. new lead compounds from microorganisms and both marine and terrestrial higher organisms. The authors focus their book on the applications and aim to provide the reader with a rapid idea on how to obtain milligram or gram quantities for pure constituents from complex mixtures with minimum effort. Descriptions of apparatus and operation procedures together with numerous examples of actual separations - often widely scattered in literature - are provided. This completely revised second edition has been enlarged by chapters on macromolecule and and enantiomer separation and on preparative GC.