Micrornas as tools in biopharmaceutical production

This book explores the uses of MicroRNAs in bioprocessing, especially in production of complex proteins in mammalian cells. Covers genomic organization, biogenesis and mode of action; miRNAs as biomarkers; manipulation of expression in cultured cells and more. MicroRNAs are small, non-coding RNA molecules that interact with target mRNAs to modulate their translation. They are now recognized as widespread and critical regulators of gene expression in the cell. This book is aimed at both industry- and academic-based researchers in the Biopharmaceutical production space who wish to learn more about these fascinating molecules. It provides an overview of what is currently known about microRNAs including: · a description of their biogenesis and mode of action · how they can be detected and measured · methods for predicting their target genes · how we can manipulate their levels in the cell · what lessons can be learned from their behaviour in cancer cells Most importantly this volume addresses how microRNAs might be utilised in the bioprocess setting to: · gain new understanding of CHO cell biology · design microRNAs expression engineering strategies to influence cell characteristics such as proliferation rate or recombinant protein productivity · predict phenotypic outcomes in cell line development projects Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Collectif (Auteur) - Verschenen op 16/11/2012 bij Springer Libri