Methods in Molecular Biology3081- RNA Remodeling Proteins

This third edition provides new and updated methods for the further study of RNA remodeling proteins. Chapters detail RNA remodeling proteins, AlphaFold to RNA-protein complexes, deep sequencing to probe transcriptome-wide remodeling events, helicase-dependent RNA structural changes, identifying helicase substrates, mapping transcriptional R-loops, leveraging techniques such as RNA-seq, DMS-seq, CLIP-seq, H-CRAC, Helicase-SELEX, proximity ligation, yeast three-hybrid assays, biochemical assays to measure RNA-protein affinity and helicase activities, structural and biophysical methods, an d functional characterization of RNA remodeling proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.