Methods in Molecular Biology3078- Salmonella

This fourth edition volume expands on the previous editions with updates on the latest methods used to study a variety of different aspects that are advancing our knowledge on Salmonella research. This fourth edition volume expands on the previous editions with updates on the latest methods used to study a variety of different aspects that are advancing our knowledge on Salmonella research. The chapters in this book cover topics such as culture-based isolation of Salmonella from different matrices; whole genome sequence analysis of Salmonella; standardized quantification of Salmonella biofilms and evaluation of antibiotic anti-biofilm activity; blocking ELISA protocol for Salmonella infection; Salmonella infection in chronic inflammation and gastrointestinal cancer; isolation of bacteriophage: an antibiotic alternative for the control of Salmonella infections; detection and characterization of Salmonella enterica serotypes by PCR technologies; and determining prevalence of Salmonella in beef. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Salmonella: Methods and Protocols, Fourth Edition is a valuable reference for both expert researchers and students. The genetic manipulation, molecular methods, and molecular imaging techniques will be of interest to geneticists, cell and molecular biologists, microbiologists, environmentalists, toxicologists, public health scientists, clinicians in human and veterinary medicine, agriculture, and other researchers who want to become familiar and apply techniques that are commonly not available in research papers. This book will also be of interest to students who are interested in learning more about the latest research on Salmonella.