Insect Genomics: Methods and Protocols

Beginning with obtaining a high-quality genome assembly using the latest sequencing data, the book continues with the identification of genome variability including single nucleotide polymorphisms and genomic inversions, and the annotation of insect genomes through genes, transposable elements, and piRNA clusters. This collection explores advances in genome sequencing and analysis tools for the understanding of insect genomes and hence their biology. Beginning with obtaining a high-quality genome assembly using the latest sequencing data, the book continues with the identification of genome variability including single nucleotide polymorphisms and genomic inversions, and the annotation of insect genomes through genes, transposable elements, and piRNA clusters. Notably, chapters also delve into downstream analyses, such as the evolution of gene families and phylogenetics, molecular biology protocols pertinent to the study of insects, as well as CRISPR-era genome editing tools to manipulate non-model insects. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice to ensure success in the laboratory. Authoritative and practical, Insect Genomics: Methods and Protocols serves as an ideal source for state-of-the-art methods and comprehensive reviews to help researchers design and implement their own studies.