Enzyme And Microbial Biosensors

The purpose of the two volumes of Protocols and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. In Enzyme and Microbial Biosensors: Protocols and Techniques, master experimentalists detail cutting-edge methods developed in their own laboratories for the construction and evaluation of enzyme- and cell-based biosensors. The enzyme biosensors detailed here are based on potentiometric, amperometric, conductimetric, optical, and thermal transducers, whereas the microbial biosensors are based on oxygen-gas electrode and optical techniques in which microorganisms are coupled to the signal transducer. All the techniques are thoroughly tested and are presented in sufficient detail to ensure robust and easily reproducible results. A companion volume, Affinity Biosensors: Protocols and Techniques, by Rogers & Mulchandani, concentrates on affinity biosensors based on optical, electrochemical, thermal, acoustic, and plasmon resource techniques as applied to nucleic acids, liposomes, and eukaryotic cells. Clearly the optimal starting point for all graduate students, postdoctoral and senior researchers, and technicians in academia, industry, and research establishments seeking rapid entry into the field of biosensors, Enzyme and Microbial Biosensors: Protocols and Techniques instantly becomes today's leading reference. Its step-by-step descriptions of various enzyme- and microbial-based biosensor techniques will allow both novices and experienced investigators alike successfully to apply these powerful new tools, and thus sharply enhance their laboratories' productivity. A cutting-edge collection of detailed, step-by-step techniques and protocols for constructing, evaluating, and using enzyme- and microbial-based biosensors. Ideal for novices starting research in their field or experienced researchers wanting to use a biosensor for a specific analytical measurement, the methods detailed here allow biochemists, analytical chemists, microbiologists, and engineers to successfully apply biosensor technology to their specific problems. The techniques include classical enzyme biosensors based on potentiometric, amperometric, conductimetric, optical, and thermal transducers, as well cell-based biosensors based on amperometric and optical transducers. A companion volume, Affinity Biosensors: Protocols and Techniques, by Rogers & Mulchandani, concentrates on cell- and affinity-based biosensors.